CPT chubhu 8ml
A Vacutainer CPT (Cell Preparation Tube) 8mL ichubhu yekuunganidza ropa yakasarudzika inoshandiswa kuparadzanisa maseru emononuclear (PBMCs) kubva muropa rose. Inowanzo shandiswa muimmunology, chirwere chinotapukira kutsvagisa, uye makiriniki mashandisirwo senge immune yekutarisa uye cell-based assays.
Zvinokosha zveVacutainer CPT Tube (8mL):
Pre-yakazadzwa ne anticoagulant uye density gradient yepakati
Iine Sodium Citrate se anticoagulant.
Inosanganisira Ficoll ™-based density gradient separation gel kuti ifambise kupatsanurwa kwemaseru emononuclear.
Inogonesa imwe-nhanho PBMC yekuzviparadzanisa nevamwe
Inobvisa kudiwa kwechinyakare bhuku density gradient centrifugation (semuenzaniso, Ficoll-Paque™).
Inoderedza nguva yekugadzirisa uye kusiyanisa.
Yakarongeka uwandu hweropa
Yakagadzirirwa kugadzirisa kusvika ku8mL yeropa rose.
Sterele, single-use chubhu
Inoshandiswa kumakiriniki uye ekutsvagisa zvikumbiro uko yakakwirira-mhando yePBMC yekuzviparadzanisa nevamwe inodiwa.
Inopindirana ne centrifugation
Imwe centrifugation protocol inodiwa kuti ubudirire kuparadzanisa maPBMC kubva kune zvimwe zvikamu zveropa.
Kushandiswa uye Zvishandiso:
PBMC kuparadzaniswa kwezvidzidzo zvekudzivirira muviri (T masero, B masero, NK masero, monocytes).
Flow cytometry, ELISPOT, cytokine assays, uye kumwe kuongorora kweimmunological.
Chirwere chetachiona uye tsvakurudzo yekudzivirira.
Autoimmune chirwere chekutarisa.
Miedzo yemakiriniki uye kuwanikwa kwebiomarker.
Mashandiro Aanoita (Basic Procedure):
1. Unganidza sampuli yeropa muCPT tube (zadza kuhuwandu hwakatarwa).
2. Inverted zvinyoronyoro (8-10 times) kusanganisa ne anticoagulant.
3. Centrifuge maererano neprotocol yemugadziri (kazhinji kutenderera 1500–1800 RCF kwemaminetsi 20–30 patembiricha yepakamuri).
4. PBMC layer mafomu pamusoro peiyo gel separator mushure me centrifugation.
5. Nyatsobvisa PBMC layer uchishandisa pipette kune yakadzika maapplications.
Kuchengeta uye Kubata:
Chengetedza panzvimbo yekushisa (15-25 ° C).
Dzivisa kupisa kwakanyanya kana kutonhora.
